Identification and Validation of Mirnas and their Targets that Regulate the Resistance Genes against Fusarium Wilt in Tomato

MicroRNAs (miRNAs) are a specialized group of small RNAs (sRNAs) that regulate gene expression in plants at both transcriptional and post-transcriptional levels. Numerous families miRNA target genes involved regulating plant immunity. In this study, we studied the role miRNAs defensive response against fungal pathogen, Fusarium oxysporum f. sp. lycopersici, which causes wilt disease tomatoes. Furthermore, patterns two novel their targets were validated by qRT-PCR. Moreover, new (miR30 miR33) further sequenced Applied Biosystems, using gene-specific primers. The results showed four miRNAs, miR33), known (miR46 miR49) differentially expressed during infection with pathogen. On other hand, (P4) (β-1,3-glucanase) an inverse correlation corresponding (miR46), (miR33, miR49), respectively. Our tomato cv. Pusa Early Dwarf is moderately susceptible to fungus because its resistance not well-expressed enough be attributed miRNAs. Sequences analysis miR30 miR33 highly conserved found different species. We predicted secondary structures minimum free energy (MFE). total miRNA30 was -1.2 -0.4 kcal/mol respectively, Vienna RNA package program V.1.7. result study could improve our comprehension play F. lycopersici. addition, it will provide sources develop resistant breeds.